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Custom Adenine Base Editor (Plasmid)
Custom Adenine Base Editor (Plasmid)
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$595
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$595
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Precision A-to-G base editing in plasmid format for transient expression or screening. Choose from single-vector designs featuring compact ABE8e variants (SpCas9, SaCas9, CjCas9, Nme2Cas9, SauriCas9) with complementary PAM specificities. Ideal for target validation, dose-response optimization, cell engineering workflows, or any application requiring flexible, non-viral delivery.
U6-(gRNA)-(pro)-ABE7.10-bGHpAExpress gRNA under U6 and ABE7.10 under selected pro for original adenine base editing with broad literature validation
- ABE7.10: First widely-adopted adenine base editor enabling A-to-G conversion with ~50% efficiency. Evolved through seven rounds for DNA substrate recognition.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-ABE8e-bGHpAExpresses sgRNA under U6 and ABE8e under selected promoter for single-AAV adenine base editing with NNGRRT PAM
- ABE8e: Enhanced ABE with 590-fold faster deamination than ABE7.10. PACE evolution enables 1.5-3× improved efficiency with broader editing window.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-SaABE8e-bGHpAExpress sgRNA under U6 and SaABE8e under selected promoter for single-AAV adenine base editing with NNGRRT PAM
- SaABE8e: Compact adenine base editor using S. aureus Cas9 (NNGRRT PAM). TadA8e deaminase provides 590-fold faster deamination than ABE7.10 with editing window at positions 4-9.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-SaABE8e-V106W-bGHpAExpress sgRNA under U6 and SaABE8e V106W under selected promoter for minimized off-target RNA editing
- SaABE8e-V106W: SaABE8e variant with V106W mutation in TadA* domain, reducing off-target RNA editing while maintaining DNA editing efficiency.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-SaKKH-ABE8e-bGHpAExpress sgRNA under U6 and SaKKH-ABE8e under selected promoter for expanded NNNRRT PAM targeting scope
- SaKHH-ABE8e: Expanded-PAM variant of SaABE8e with KKH mutations (E782K/N968K/R1015H) enabling NNNRRT PAM recognition.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-CjABE8e-bGHpAExpresses sgRNA under U6 and CjABE8e under selected promoter for ultra-compact single-AAV delivery
- CjABE8e: Ultra-compact adenine base editor using Campylobacter jejuni Cas9. Smallest ABE architecture (~3.8 kb) with 22-nt protospacer and editing window at positions 3-11.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-Nme2ABE8e-bGHpAExpress sgRNA under U6 and Nme2ABE8e under selected promoter for A/T-rich region targeting with N4CC PAM
- Nme2ABE8e: Ultra-compact adenine base editor using Nme2Cas9 with N4CC PAM, enabling targeting of A-rich sequences.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-SauriABE8e-bGHpAExpress sgRNA under U6 and SauriABE8e under selected promoter for alternative PAM specificity and genome coverage
- SauriABE8e: Compact adenine base editor using S. auricularis Cas9, providing alternative PAM specificity for expanded targeting.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
Deliverables
- Titer: 1 µg/mL or higher
- Delivery Timeline: 12–14 days
Specifications
High copy plasmid DNA produced in E. coli using the proprietary Ambryon backbone with pMB1 origin of replication and ampicillin resistance. Bacteria are cultured in LB medium at 37°C and harvested at late exponential phase. Cells are lysed by alkaline lysis and purified by endotoxin-free anion-exchange chromatography. Final DNA is resuspended in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) and quality-verified by A260/A280 spectrophotometry (≥1.8) and sequence confirmation. Store at -20°C and avoid repeated freeze-thaw cycles. Ships at ambient temperature.
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