S. auricularis ABE8e Adenine Base Editor

Characteristics

Compact architecture using SauriCas9 (similar size to SaCas9) enables single-AAV delivery. Uses 21-nucleotide protospacer. Editing window spans positions 4-9. Alternative PAM specificity complements SaCas9 and other compact editors. Part of three-editor suite collectively targeting ~82% of genomic adenines. Compatible with SaCas9 sgRNA scaffolds.

Applications: Single-AAV base editing at sites inaccessible to SaCas9, Nme2Cas9, and CjCas9 PAM requirements. Complementary to other compact editors for comprehensive genome coverage. Enables multi-editor therapeutic strategies where different PAM specificities are required for targeting multiple loci.

Limitations: Specific PAM requirements limit individual targeting scope. Less characterized than SaCas9-based editors. May require optimization of guide design and delivery parameters for specific applications.

Architecture: All-in-one (single vector)

References

1. Gaudelli NM, Komor AC, Rees HA, et al. (2017). Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464-471 - Gaudelli 2017 Adenine Base Editor
2. Richter et al. (2020). Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Nat Biotechnol - Richter 2020 ABE8e
3. Davis et al. (2022). Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors. Nat Biomed Eng - Davis 2022 Single-AAV ABEs
4. Yang et al. (2024). Adenine base editor-based correction of the cardiac pathogenic Lmna c.1621C > T mutation in murine hearts. J Cell Mol Med - Yang 2024 SauriABE8e