Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

Richter et al. (2020). Nat Biotechnol DOI: 10.1038/s41587-020-0453-z Citations: 1087

Parts used

ABE8e Base Editor

Key findings

  • PACE evolution generated TadA-8e with eight mutations conferring 590-fold increased deamination rate versus ABE7.10. ABE8e shows substantially improved editing with diverse Cas9 and Cas12 variants, enabling multiplex and regulatory region editing.