Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

Gaudelli NM, Komor AC, Rees HA, et al. (2017). Nature 551:464-471 | DOI: 10.1038/nature24644 | Citations: 4827

Key Finding

Developed adenine base editor (ABE) enabling A-to-G conversion. Evolved TadA deaminase fused to Cas9 for targeting adenine bases. Complemented CBE technology to enable correction of all four transition mutations.

Parts Used