U6-(gRNA)-(pro)-ABE7.10-bGHpA
Express gRNA under U6 and ABE7.10 under selected pro for original adenine base editing with broad literature validation
Characteristics
Original adenine base editor with wild-type TadA and evolved TadA* domains fused to SpCas9 D10A nickase. Editing window at protospacer positions 4-8. Extensively validated across organisms and cell types. NGG PAM requirement. Foundation for all subsequent ABE variants.
Parts
Custom Inserts
SpCas9 gRNA target
(20-23 bp)
Literature Sources
- Gaudelli NM, Komor AC, Rees HA, et al. (2017). Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464-471.
Available Products
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Custom Adenine Base Editor (Plasmid)
Regular price From $595Regular priceUnit price per
Additional Information
Applications: Well-characterized targets with established ABE7.10 protocols. Research applications where extensive literature precedent exists. When published data specifically used ABE7.10. Target validation before optimizing with ABE8e variants.