U6-(gRNA)-(pro)-SaABE8e-V106W-bGHpA
Express sgRNA under U6 and SaABE8e V106W under selected promoter for minimized off-target RNA editing
Characteristics
V106W mutation in TadA* domain reduces off-target RNA editing 7.2-fold (from 0.49% to 0.068% average frequency) while maintaining robust DNA editing. Provides 2.7-fold improvement in off-target DNA editing specificity and 3.7-fold reduction in indel formation compared to parent SaABE8e. NNGRRT PAM recognition.
Parts
Custom Inserts
SaCas9 gRNA Target
(21-23 bp)
Literature Sources
- Rees et al. (2019). Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv.
- Davis et al. (2022). Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors. Nat Biomed Eng.
Available Products
-
Custom Adenine Base Editor (Plasmid)
Regular price From $595Regular priceUnit price per -
Custom Adenine Base Editor (AAV)
Regular price From $1,995Regular priceUnit price per
Additional Information
Applications: Therapeutic development requiring minimal transcriptome perturbation and highest specificity. Long-term expression applications where cumulative RNA editing is a concern. When target validation or clinical translation demands lowest possible off-target effects across DNA and RNA.