U6-(gRNA)-(pro)-SaABE8e-V106W-bGHpA

Express sgRNA under U6 and SaABE8e V106W under selected promoter for minimized off-target RNA editing

Characteristics

V106W mutation in TadA* domain reduces off-target RNA editing 7.2-fold (from 0.49% to 0.068% average frequency) while maintaining robust DNA editing. Provides 2.7-fold improvement in off-target DNA editing specificity and 3.7-fold reduction in indel formation compared to parent SaABE8e. NNGRRT PAM recognition.

Parts

SaABE8e-V106W Base Editor bGH PolyA Signal U6 Promoter

Custom Inserts

SaCas9 gRNA Target (21-23 bp)

Literature Sources

  1. Rees et al. (2019). Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv- Rees 2019 RNA Editing Minimization
  2. Davis et al. (2022). Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors. Nat Biomed Eng- Davis 2022 Single-AAV ABEs

Available Products

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Additional Information

Applications: Therapeutic development requiring minimal transcriptome perturbation and highest specificity. Long-term expression applications where cumulative RNA editing is a concern. When target validation or clinical translation demands lowest possible off-target effects across DNA and RNA.

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