U6-(gRNA)-(pro)-Nme2ABE8e-bGHpA

Express sgRNA under U6 and Nme2ABE8e under selected promoter for A/T-rich region targeting with N4CC PAM

Characteristics

Recognizes N4CC PAM (N=any nucleotide), enabling access to A/T-rich genomic regions inaccessible to GC-rich PAM editors. Uses 24-nucleotide protospacer. Editing window spans positions 4-10. Compact Neisseria meningitidis Cas9 architecture (~4.2 kb) provides ample cargo space for regulatory elements.

Parts

Nme2ABE8e Base Editor bGH PolyA Signal U6 Promoter

Custom Inserts

Nme2Cas9 gRNA Target (24-24 bp)

Literature Sources

  1. Davis et al. (2022). Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors. Nat Biomed Eng- Davis 2022 Single-AAV ABEs

Available Products

No products currently available with this design. Contact us for custom orders.

Additional Information

Applications: Targeting A/T-rich genomic regions with N4CC PAM sequences. Complementary to SaCas9 and CjCas9 variants for achieving comprehensive genome coverage. Multi-editor therapeutic strategies where different PAM specificities are required. Part of suite collectively accessing ~82% of genomic adenines.

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