U6-(gRNA)-(pro)-SaKKH-ABE8e-bGHpA
Express sgRNA under U6 and SaKKH-ABE8e under selected promoter for expanded NNNRRT PAM targeting scope
Characteristics
KKH mutations (E782K/N968K/R1015H) expand PAM recognition from NNGRRT to NNNRRT, significantly increasing targetable sites. Achieved highest therapeutic editing in Davis 2022 study with 93% knockdown of PCSK9/Pcsk9/Angptl3. Superior on-target activity compared to standard NNGRRT PAM variant. Editing window at positions 4-9.
Parts
Custom Inserts
SaCas9-KKH gRNA Target
(21-23 bp)
Literature Sources
- Davis et al. (2022). Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors. Nat Biomed Eng.
Available Products
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Custom Adenine Base Editor (Plasmid)
Regular price From $595Regular priceUnit price per -
Custom Adenine Base Editor (AAV)
Regular price From $1,995Regular priceUnit price per
Additional Information
Applications: Maximum targeting flexibility with single-AAV SaCas9 platform. Preferred variant for therapeutic applications requiring robust editing. Validated for lipid-lowering gene therapy via PCSK9 and ANGPTL3 disruption. Use when target sites contain NNNRRT PAMs that exclude standard NNGRRT sequences.