U6-(gRNA)-(pro)-ABE8e-bGHpA

Expresses sgRNA under U6 and ABE8e under selected promoter for single-AAV adenine base editing with NNGRRT PAM

Parts

ABE8e Base Editor bGH PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

  1. Richter et al. (2020). Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Nat Biotechnol- Richter 2020 ABE8e
  2. Gaudelli et al. (2020). Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol- Gaudelli 2020 ABE8 Variants

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