U6-(gRNA)-(pro)-ABE8e-bGHpA

Expresses sgRNA under U6 and ABE8e under selected promoter for single-AAV adenine base editing with NNGRRT PAM

Parts

ABE8e bGH pA U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

  1. Richter et al. (2020). Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Nat Biotechnol.
  2. Gaudelli et al. (2020). Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol.

Available Products