Directed evolution of adenine base editors with increased activity and therapeutic application

Gaudelli et al. (2020). Nat Biotechnol | DOI: 10.1038/s41587-020-0491-6 | Citations: 665

Key Finding

Library-based evolution of TadA-7.10 generated 40 ABE8 variants. ABE8s show 1.5× higher editing at A5-A7 and 3.2× at A3-A4/A8-A10 positions versus ABE7.10. Achieved 98-99% target modification in primary human T cells with reduced off-target effects.

Parts Used

ABE8e