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SpCas9 Dual-AAV Editor

SpCas9 Dual-AAV Editor

AAV-SPCAS9-CMV-XS

$2,495
$2,495
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Promoter
Scale

Dual-vector SpCas9 system for maximum payload flexibility. Separates nuclease and guide RNA into independent AAVs, enabling incorporation of tissue-specific promoters, multiple sgRNAs, and extended regulatory elements without size constraints. Wild-type SpCas9 (1368aa) with NGG PAM recognition delivers established performance across diverse applications. Requires co-transduction of both vectors for editing activity. Based on validated Zhang lab dual-AAV architecture (Addgene pX551/pX552)1.

 

Specifications

Single stranded AAV produced in 293T cells and purified through two (2) rounds of CsCl ultracentrifugation and stored in 0.001% F-68 in DPBS with additional 150 mM NaCl. Ships on dry ice overnight.

Deliverables

  • Titer: 1e13 VG/mL or higher
  • Full Capsid Ratio: 0.85 or higher
  • Delivery Timeline: 18–21 days

Available Serotypes

This construct can be packaged in hundreds of AAV serotypes to target your specific tissue. We offer all standard serotypes (AAV1-9) plus specialized variants for muscle (MyoAAV-2A, MyoAAV-1A, AAVMyo), brain (PHP.eB, AAV9P31, AAV2-retro), and clinical applications (AAV2, Spark100). Other popular options include AAV-rh.74, AAV6, AAV6.2FF, and AAV3. Browse our complete serotype library or contact us for recommendations.

Parts

  • SpCas9 Nuclease: Most widely studied CRISPR nuclease. High efficiency across diverse cell types and organisms. Requires dual-AAV system for in vivo delivery due to size. Extensive validation data available. Multiple engineered variants exist for enhanced specificity.
  • CMV Promoter: Strong, ubiquitous expression across most mammalian cell types. Provides highest initial transgene levels but may experience silencing in some contexts over time.
  • EFS Promoter: Compact promoter derived from human elongation factor 1-alpha. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV.
  • hSyn Promoter: Neuron-specific promoter restricting expression to neurons. Minimal activity in glial cells. Compact size suitable for AAV packaging.
  • hPGK Promoter: Moderate, stable expression with lower immunogenicity than viral promoters. Maintains consistent levels over time without significant silencing.
  • U6 Promoter: RNA Pol III promoter for expressing short RNAs like guide RNAs, shRNAs, and other small non-coding RNAs. Not for protein-coding genes.

References

  1. In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9, Swiech et al. (2015) Nat Biotechnol 33:102-106
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