AAV2-Retro Serotype

Engineered AAV2 capsid with 100-fold enhanced retrograde transport efficiency, delivering transgenes to projection neurons by infecting axon terminals and trafficking to the soma. Does not cross synapses, enabling input-specific circuit labeling without contamination from neighboring pathways. Standard vector for optogenetics, chemogenetics, and long-range neural connectivity studies.

Length: 1 bp

Tropism: Neurons (retrograde transport), axonal terminals to soma

Characteristics

Engineered via directed evolution with 10-amino acid capsid insertion (LADQDYTKTA). 100-fold improvement in retrograde transport efficiency versus AAV2. Enables efficient gene delivery to projection neurons via axon terminals. Revolutionized circuit mapping techniques. Maintains neuronal tropism. Does not cross synapses.

Applications

Neural circuit mapping and tracing. Projection neuron targeting in neuroscience research. Optogenetics and chemogenetics in defined circuits. Long-range connectivity studies. Retrograde labeling tool. Standard neuroscience research vector.

Literature References

  1. Tervo et al. (2016). A designer AAV variant permits efficient retrograde access to projection neurons. Neuron - Tervo 2016 AAV2-retro

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