AAV2-Retro Serotype
Engineered AAV2 capsid with 100-fold enhanced retrograde transport efficiency, delivering transgenes to projection neurons by infecting axon terminals and trafficking to the soma. Does not cross synapses, enabling input-specific circuit labeling without contamination from neighboring pathways. Standard vector for optogenetics, chemogenetics, and long-range neural connectivity studies.
Characteristics
Engineered via directed evolution with 10-amino acid capsid insertion (LADQDYTKTA). 100-fold improvement in retrograde transport efficiency versus AAV2. Enables efficient gene delivery to projection neurons via axon terminals. Revolutionized circuit mapping techniques. Maintains neuronal tropism. Does not cross synapses.
Applications: Neural circuit mapping and tracing. Projection neuron targeting in neuroscience research. Optogenetics and chemogenetics in defined circuits. Long-range connectivity studies. Retrograde labeling tool. Standard neuroscience research vector.
References
- Tervo et al. (2016). A designer AAV variant permits efficient retrograde access to projection neurons. Neuron - Tervo 2016 AAV2-retro
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