SpCas9 Nuclease

Length: 4100 bp(1366 aa)

PAM: NGG

Origin: Streptococcus pyogenes

Characteristics

Most widely studied CRISPR nuclease. High efficiency across diverse cell types and organisms. Requires dual-AAV system for in vivo delivery due to size. Extensive validation data available. Multiple engineered variants exist for enhanced specificity.

Literature References

  1. Cong et al. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science - Cong 2013 SpCas9
  2. Jinek et al. (2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science - Jinek 2012 SpCas9

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