(pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-mCherry-bGHpA

Dual-gRNA SpCas9 companion vector with CAG-driven mCherry for broad fluorescent verification of transduced cells.

Characteristics

Expresses two gRNAs from tandem U6 promoters. CAG-driven mCherry provides ubiquitous red-channel whole-cell fluorescence without nuclear membrane anchoring. Smaller payload than KASH-containing variants.

Parts

SpCas9 Nuclease mCherry CAG Promoter bGH PolyA Signal SV40 Late PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Related Designs

(pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-NLS-EGFP-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-EGFP-KASH-bGHpA

Literature Sources

    Available Products

    No products currently available with this design. Contact us for custom orders.

    Additional Information

    Applications: Dual-target CRISPR editing with whole-cell red fluorescent verification. Preferred when FANS nuclear sorting is not needed and red channel is available. Compatible with GFP-tagged proteins or green channel imaging in the same experiment.

    Images