(pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA

Dual-gRNA SpCas9 companion vector with CAG-driven EGFP-KASH for FANS-compatible nuclear envelope labeling of transduced cells.

Characteristics

Expresses two independent gRNAs from tandem U6 promoters for simultaneous targeting of two genomic loci. CAG-driven EGFP-KASH localizes to the outer nuclear membrane, enabling FANS-based isolation of transduced nuclei without cell dissociation. Ubiquitous CAG expression ensures reporter signal in any cell type.

Parts

SpCas9 Nuclease EGFP KASH Domain CAG Promoter bGH PolyA Signal SV40 Late PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Related Designs

U6-(SaCas9gRNA)-EFS-EGFP-KASH-hGHpA (pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-NLS-EGFP-bGHpA (pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-EGFP-KASH-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + 3*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA

Literature Sources

  1. Kumar et al. (2018). The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Front Mol Neurosci- Kumar 2018 In Vivo CRISPR

Available Products

No products currently available with this design. Contact us for custom orders.

Additional Information

Applications: Multiplex 2-target CRISPR editing paired with a SaCas9 or SpCas9 vector. Preferred when nuclei must be isolated post-editing for downstream single-nucleus genomics (snRNA-seq, snATAC-seq). EGFP channel allows flow-based gating.

Images