(pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-mCherry-bGHpA

Single-gRNA SpCas9 companion vector with CAG-driven mCherry for broad fluorescent verification of transduced cells.

Characteristics

Expresses one gRNA from U6. CAG-driven mCherry provides ubiquitous red-channel whole-cell fluorescence. Minimal design — single gRNA and a small reporter with no nuclear targeting sequences. Compact payload.

Parts

mCherry CAG Promoter bGH PolyA Signal SV40 Late PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Related Designs

(pro)-SpCas9-SV40pA + U6-(gRNA)-hSyn-mCherry-bGHpA

Literature Sources

    Available Products

    No products currently available with this design. Contact us for custom orders.

    Additional Information

    Applications: Single-target CRISPR editing with red-channel transduction verification by imaging or whole-cell FACS. Green channel is free for co-reporters or imaging probes. Suitable for most cell culture and in vivo contexts where FANS is not required.

    Images