(pro)-SpCas9-SV40pA + U6-(gRNA)-hSyn-mCherry-bGHpA

Single-gRNA SpCas9 companion vector with neuron-specific hSyn-driven mCherry for fluorescent verification of transduction in neuronal cells.

Characteristics

Expresses one gRNA from U6. hSyn promoter restricts mCherry expression to neurons, providing red-channel whole-cell fluorescence specifically in neuronal populations within mixed-cell tissue. No nuclear membrane targeting.

Parts

SpCas9 Nuclease mCherry hSyn Promoter bGH PolyA Signal SV40 Late PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Related Designs

(pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-EGFP-KASH-bGHpA (pro)-SpCas9-SV40pA + 3*{U6-(gRNA)}-hSyn-mCherry-KASH-bGHpA

Literature Sources

    Available Products

    No products currently available with this design. Contact us for custom orders.

    Additional Information

    Applications: Single-target neuronal CRISPR editing in vivo where cell-type-restricted reporter expression is required. Preferred for brain tissue experiments — only neurons fluoresce, enabling visual confirmation of cell-type specificity without FANS. Compatible with GFP channel for co-labeling.

    Images