(pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-EGFP-KASH-bGHpA

Single-gRNA SpCas9 companion vector with CAG-driven EGFP-KASH for FANS-compatible nuclear envelope labeling of transduced cells.

Characteristics

Expresses one gRNA from U6. CAG-driven EGFP-KASH localizes to the outer nuclear membrane, enabling FANS-based isolation of transduced nuclei. Functionally equivalent to the Kumar 2018 design with a ubiquitous promoter. Smallest payload in the EGFP-KASH family.

Parts

SpCas9 Nuclease CAG Promoter EGFP KASH Domain bGH PolyA Signal SV40 Late PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Related Designs

U6-(SaCas9gRNA)-EFS-EGFP-KASH-hGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-hSyn-mCherry-bGHpA (pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-(ins)

Literature Sources

  1. Kumar et al. (2018). The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Front Mol Neurosci- Kumar 2018 In Vivo CRISPR

Available Products

No products currently available with this design. Contact us for custom orders.

Additional Information

Applications: Single-target CRISPR editing where nuclei must be sorted post-transduction for downstream single-nucleus omics. Most compact FANS-compatible design in this series — preferred when AAV payload is constrained.

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