(pro)-SpCas9-SV40pA + U6-(gRNA)-(ins)
Minimal single-gRNA SpCas9 companion vector with a custom payload slot for co-delivery of an additional sequence element.
Characteristics
Expresses one gRNA from U6. Contains no fluorescent reporter — the insert slot is the primary functional addition beyond the gRNA, intended for a user-defined element such as an HDR template, second cargo gene, or regulatory sequence. Minimal footprint maximizes insert capacity.
Custom Inserts
Related Designs
Literature Sources
Available Products
No products currently available with this design. Contact us for custom orders.
Additional Information
Applications: Single-target CRISPR editing requiring co-packaging of a custom sequence (e.g., HDR donor template, transgene, or additional expression cassette) in the same vector as the gRNA. Appropriate when no transduction reporter is needed or the insert itself provides a detectable marker.
Fixed sequence length: 350 bp