(pro)-SpCas9-SV40pA + 3*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA

Triple-gRNA SpCas9 companion vector with CAG-driven EGFP-KASH for FANS-compatible nuclear envelope labeling of transduced cells.

Characteristics

Expresses three independent gRNAs from tandem U6 promoters enabling simultaneous targeting of three genomic loci. CAG-driven EGFP-KASH marks the outer nuclear membrane for FANS-based nuclei isolation. Ubiquitous reporter expression is cell-type agnostic.

Parts

SpCas9 Nuclease EGFP KASH Domain CAG Promoter bGH PolyA Signal SV40 Late PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Related Designs

U6-(SaCas9gRNA)-EFS-EGFP-KASH-hGHpA (pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + 3*{U6-(gRNA)}-CAG-mCherry-bGHpA

Literature Sources

  1. Kumar et al. (2018). The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Front Mol Neurosci- Kumar 2018 In Vivo CRISPR

Available Products

No products currently available with this design. Contact us for custom orders.

Additional Information

Applications: High-multiplicity CRISPR editing requiring three simultaneous guide RNAs — e.g., targeting multiple exons of one gene, or disrupting members of a gene family. FANS enables downstream nuclei-based omics on edited cells.

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