(pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-NLS-EGFP-bGHpA

Dual-gRNA SpCas9 companion vector with CAG-driven NLS-EGFP for nuclear-localized green fluorescent verification of transduced cells.

Characteristics

Expresses two gRNAs from tandem U6 promoters. CAG-driven NLS-EGFP concentrates green fluorescence in the nucleus for clear imaging-based transduction verification and nuclear segmentation. Unlike EGFP-KASH, signal fills the nucleoplasm rather than decorating the nuclear envelope.

Parts

SpCas9 Nuclease EGFP SV40 NLS CAG Promoter bGH PolyA Signal SV40 Late PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp) SpCas9 gRNA target (20-23 bp)

Related Designs

(pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA (pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-mCherry-bGHpA (pro)-SpCas9-SV40pA + U6-(gRNA)-CAG-EGFP-KASH-bGHpA

Literature Sources

    Available Products

    No products currently available with this design. Contact us for custom orders.

    Additional Information

    Applications: Dual-target editing where nuclear-localized fluorescence improves imaging clarity over cytoplasmic reporters — useful for automated cell counting, nuclear segmentation in high-content imaging, or distinguishing signal from cytoplasmic autofluorescence. Not designed for FANS sorting.

    Images