(pro)-OpenCRISPR-1-SV40pA + 2*{U6-(gRNA)}-CAG-mCherry-bGHpA

OpenCRISPR-1 counterpart to the SpCas9 dual-gRNA CAG-mCherry design. Nuclease AAV expresses AI-designed OpenCRISPR-1 under a custom promoter with SV40pA; companion AAV delivers two U6-driven gRNAs and CAG-mCherry for whole-cell red fluorescence verification. Canonical SpCas9 sgRNAs are fully compatible.

Characteristics

OpenCRISPR-1 nuclease with 95% off-target reduction; particularly valuable in dual-target designs where off-target risk accumulates across both cut sites. CAG-mCherry provides ubiquitous red-channel whole-cell fluorescence. Not FANS-compatible. Green channel preserved for co-reporters.

Parts

OpenCRISPR-1 Nuclease mCherry CAG Promoter bGH PolyA Signal SV40 Late PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Related Designs

(pro)-SpCas9-SV40pA + 2*{U6-(gRNA)}-CAG-mCherry-bGHpA (pro)-OpenCRISPR-1-SV40pA + 2*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA (pro)-OpenCRISPR-1-SV40pA + U6-(gRNA)-CAG-mCherry-bGHpA

Literature Sources

  1. Ruffolo et al. (2025). Design of highly functional genome editors by modelling CRISPR–Cas sequences. Nature- Ruffolo 2025 OpenCRISPR
  2. Platt et al. (2014). CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Cell- Platt 2014 Cas9 Mice

Available Products

No products currently available with this design. Contact us for custom orders.

Additional Information

Validated in: HEK293 cells (OpenCRISPR-1 characterization); dual-gRNA companion architecture validated in mammalian cells

Applications: Dual-target editing with whole-cell fluorescence readout where FANS is not required. Upgrade from SpCas9 counterpart when specificity or immunogenicity is a concern. Existing validated SpCas9 gRNA pairs are reusable.

Images