CRISPR-Cas9 knockin mice for genome editing and cancer modeling
Key findings
Generated first Cre-dependent Cas9 knockin mouse (Rosa26-LSL-Cas9). Enabled sgRNA-only AAV delivery for tissue-specific editing without dual vectors. Demonstrated multiplexed cancer modeling by simultaneously targeting Kras, p53, and Lkb1 with single AAV vector (AAV-KPL) containing three sgRNAs plus Cre recombinase.