(pro)-OpenCRISPR-1-SV40pA + 2*{U6-(gRNA)}-CAG-EGFP-KASH-bGHpA
OpenCRISPR-1 counterpart to the SpCas9 dual-gRNA CAG-EGFP-KASH design. Nuclease AAV expresses AI-designed OpenCRISPR-1 under a custom promoter with SV40pA; companion AAV delivers two U6-driven gRNAs and CAG-EGFP-KASH for FANS-compatible nuclear envelope labeling. Canonical SpCas9 sgRNAs are fully compatible — the companion AAV is architecturally identical to its SpCas9 counterpart.
Characteristics
OpenCRISPR-1 nuclease enables dual-locus targeting with 95% fewer off-target edits compared to SpCas9 — particularly advantageous in multiplexed designs where cumulative off-target risk compounds across multiple cut sites. Tandem U6 cassettes carry recombination risk during AAV production; same precautions apply as SpCas9 counterpart. CAG-EGFP-KASH enables FANS.
Custom Inserts
Related Designs
Literature Sources
- Ruffolo et al. (2025). Design of highly functional genome editors by modelling CRISPR–Cas sequences. Nature- Ruffolo 2025 OpenCRISPR
- Kumar et al. (2018). The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Front Mol Neurosci- Kumar 2018 In Vivo CRISPR
- Platt et al. (2014). CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Cell- Platt 2014 Cas9 Mice
Available Products
No products currently available with this design. Contact us for custom orders.
Additional Information
Validated in: HEK293 cells (OpenCRISPR-1 characterization); dual-gRNA companion architecture validated in mammalian cells
Applications: Multiplexed 2-target editing where reduced cumulative off-target risk is a priority. Direct upgrade path from the SpCas9 dual-gRNA counterpart when improved specificity or immunogenicity profile is needed. Existing validated SpCas9 gRNA pairs are reusable without redesign.