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Custom Cytosine Base Editor (Dual AAV)
Custom Cytosine Base Editor (Dual AAV)
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$2,295
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Precision C-to-T base editing via dual-AAV split-intein delivery for in vivo applications. Choose from SpCas9-based (BE3) or SaCas9-based (saCBE, saBE3.9max) systems with standard or KKH-enhanced PAM recognition. Reconstitutes in target cells from co-transduced N-terminal and C-terminal AAV vectors. Ideal for liver, muscle, and CNS applications requiring cytosine editing with flexible PAM targeting (NGG, NNGRRT, or NNNRRT).
U6-(gRNA)-(pro)-CBE-NT-bGHpA + EFS-CBE-CT-bGHpATwo-vector system delivering CBE N-terminal and C-terminal split-intein fragments for reconstituted BE3 cytosine base editing
- CBE N-term: N-terminal fragment of cytosine base editor split via Npu intein at E573. Requires C-terminal counterpart for reconstitution.
- CBE C-term: C-terminal fragment of cytosine base editor split via Npu intein at C574. Pairs with N-terminal fragment for editor reconstitution.
- EFS Promoter: Compact promoter derived from human elongation factor 1-alpha. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-SaCBE-NT-bGHpA + EFS-SaCBE-CT-bGHpATwo-vector system delivering S. aureus CBE split-intein fragments with NNGRRT PAM recognition
- SaCBE N-term: N-terminal fragment of SaCas9-based cytosine editor split via Npu intein at Lys534. Compact size enables efficient AAV packaging.
- SaCBE C-term: Compact SaCas9-based cytosine editor split via Npu intein for dual-AAV delivery. Smaller size than SpCas9 enables efficient packaging.
- EFS Promoter: Compact promoter derived from human elongation factor 1-alpha. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-SaCBE-NT-bGHpA + EFS-SaCBE-KKH-CT-bGHpATwo-vector system with SaCBE N-terminal and KKH-enhanced C-terminal for expanded NNNRRT PAM targeting
- SaCBE-KKH C-term: SaCas9-KKH variant base editor with altered PAM recognition (NNNRRT). Enables broader targeting when combined with compact AAV packaging.
- SaCBE N-term: N-terminal fragment of SaCas9-based cytosine editor split via Npu intein at Lys534. Compact size enables efficient AAV packaging.
- EFS Promoter: Compact promoter derived from human elongation factor 1-alpha. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-SaBE39max-NT-bGHpA + EFS-SaBE39max-CT-bGHpATwo-vector system delivering optimized S. aureus BE3.9max split-intein fragments with codon-optimized APOBEC1
- SaBE3.9max N-term: N-terminal fragment of optimized SaCas9 base editor split via Npu intein at Lys534. Combines compact size with enhanced expression.
- SaBE3.9max C-term: C-terminal fragment of optimized SaCas9 base editor split via Npu intein at Cys535. Pairs with N-terminal for high-efficiency editing.
- EFS Promoter: Compact promoter derived from human elongation factor 1-alpha. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
U6-(gRNA)-(pro)-SaBE39max-NT-bGHpA + EFS-SaBE39max-KKH-CT-bGHpATwo-vector system with optimized SaBE3.9max N-terminal and KKH-enhanced C-terminal for maximum efficiency and PAM flexibility
- SaBE3.9max N-term: N-terminal fragment of optimized SaCas9 base editor split via Npu intein at Lys534. Combines compact size with enhanced expression.
- SaBE3.9max-KKH C-term: Optimized SaCas9-KKH base editor with enhanced nuclear localization and NNNRRT PAM. Combines BE3.9max improvements with broader targeting.
- EFS Promoter: Compact promoter derived from human elongation factor 1-alpha. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
Deliverables
- Titer: 1e13 VG/mL or higher
- Full Capsid Ratio: 0.85 or higher
- Delivery Timeline: 18–21 days
Specifications
Single stranded AAV produced in 293T cells and purified through two (2) rounds of CsCl ultracentrifugation and stored in 0.001% F-68 in DPBS with additional 150 mM NaCl. Ships on dry ice overnight.
This construct can be packaged in hundreds of AAV serotypes to target your specific tissue. We offer all standard serotypes (AAV1-9) plus specialized variants for muscle (MyoAAV-2A, MyoAAV-1A, AAVMyo), brain (PHP.eB, AAV9P31, AAV2-retro), and clinical applications (AAV2, Spark100). Other popular options include AAV-rh.74, AAV6, AAV6.2FF, and AAV3. Browse our complete serotype library or contact us for recommendations.
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