U6-(gRNA)-(pro)-SaBE39max-NT-bGHpA + EFS-SaBE39max-CT-bGHpA

Two-vector system delivering optimized S. aureus BE3.9max split-intein fragments with codon-optimized APOBEC1

Characteristics

First AAV delivers SaBE3.9max N-terminal (codon-optimized APOBEC1 + enhanced NLS + SaCas9 aa1-534) under EFS promoter. Second AAV delivers SaBE3.9max C-terminal (SaCas9 aa535-1053) under selected promoter with U6-gRNA. Split at Lys534/Cys535 using Npu DnaE intein. Reconstitutes full SaBE3.9max activity with improved expression and efficiency. NNGRRT PAM recognition.

Parts

SaBE3.9max N-term SaBE3.9max C-term EFS Promoter bGH pA U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

Levy et al. (2020). Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng.

Available Products

Custom Cytosine Base Editor (Dual AAV)

Custom Cytosine Base Editor (Dual AAV)

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Additional Information

Applications: Enhanced cytosine editing efficiency with SaCas9 platform via dual-AAV. When optimization and higher expression levels improve therapeutic outcomes. Alternative to standard SaCBE for challenging targets.