U6-(gRNA)-(pro)-SaBE39max-NT-bGHpA + EFS-SaBE39max-KKH-CT-bGHpA

Two-vector system with optimized SaBE3.9max N-terminal and KKH-enhanced C-terminal for maximum efficiency and PAM flexibility

Characteristics

First AAV delivers SaBE3.9max N-terminal (codon-optimized APOBEC1 + enhanced NLS + SaCas9 aa1-534) under EFS promoter. Second AAV delivers SaBE3.9max-KKH C-terminal (SaCas9 aa535-1053 with E782K/N968K/R1015H mutations) under selected promoter with U6-gRNA. Combines optimization with PAM expansion (NNNRRT). Split at Lys534/Cys535 using Npu DnaE intein. Highest activity SaCas9 CBE variant with broadest PAM compatibility.

Parts

SaBE3.9max N-term SaBE3.9max-KKH C-term EFS Promoter bGH pA U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

Levy et al. (2020). Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng.

Available Products

Custom Cytosine Base Editor (Dual AAV)

Custom Cytosine Base Editor (Dual AAV)

Regular price From $2,295

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Additional Information

Applications: Maximum cytosine editing performance via dual-AAV when both optimization and expanded PAM are beneficial. Challenging therapeutic targets requiring highest possible editing rates with NNNRRT PAM access.