Custom Cytosine Base Editor (Plasmid)
Custom Cytosine Base Editor (Plasmid)
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Precision C-to-T base editing in plasmid format for transient expression or screening. Choose from validated CBE variants (BE3, BE4max, AncBE4max, Target-AID) optimized for different activity profiles and specificity requirements. Ideal for introducing stop codons, disrupting splice sites, correcting G-to-A pathogenic mutations, or target validation before committing to viral delivery. Flexible non-viral format for cell engineering and research applications.
- BE3: First-generation cytosine base editor achieving >30% C-to-T conversion without double-strand breaks. Foundation for all subsequent CBE development.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
- BE4max: Optimized cytosine base editor with 1.8× higher efficiency than BE4. Enhanced nuclear import and codon optimization boost C-to-T conversion.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
- AncBE4max: Advanced CBE using ancestral APOBEC reconstruction. Minimized transcriptome-wide off-targets with narrower editing window and lower toxicity.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
- Target-AID: Alternative cytosine base editor using sea lamprey cytidine deaminase. Narrower editing window (positions 2-4) with reduced cellular toxicity.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
Deliverables
- Titer: 1 µg/mL or higher
- Delivery Timeline: 12–14 days
Specifications
High copy plasmid DNA produced in E. coli using the proprietary Ambryon backbone with pMB1 origin of replication and ampicillin resistance. Bacteria are cultured in LB medium at 37°C and harvested at late exponential phase. Cells are lysed by alkaline lysis and purified by endotoxin-free anion-exchange chromatography. Final DNA is resuspended in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) and quality-verified by A260/A280 spectrophotometry (≥1.8) and sequence confirmation. Store at -20°C and avoid repeated freeze-thaw cycles. Ships at ambient temperature.
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