U6-(gRNA)-(pro)-BE4max-bGHpA

Express gRNA under U6 and BE4max under selected promoter for enhanced cytosine editing efficiency and reduced indels

Characteristics

Codon-optimized BE4 variant with enhanced nuclear localization signals for improved expression and efficiency. Contains dual UGI domains to reduce indel formation and increase product purity. Editing window at positions 4-8 with NGG PAM. Achieves higher editing rates than BE3 while reducing unwanted by-products. Requires dual-AAV packaging.

Parts

BE4max bGH pA U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

  1. Koblan et al. (2018). Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol.

Available Products

Additional Information

Applications: Therapeutic applications requiring maximum C-to-T editing efficiency with minimal indels. In vivo gene therapy where high editing rates are critical. Introduction of stop codons for gene inactivation. Correction of G-to-A pathogenic mutations. Preferred over BE3 when optimization and purity matter more than literature precedent.

Fixed sequence length: 6100 bp