U6-(gRNA)-(pro)-AncBE4max-bGHpA

Express gRNA under U6 and AncBE4max under selected promoter for thermostable cytosine editing with enhanced in vivo stability

Characteristics

Uses ancestrally reconstructed APOBEC1 with enhanced thermostability at physiological temperature (37°C). Maintains high editing efficiency while providing superior protein stability for in vivo applications. Codon-optimized with dual UGI domains. Editing window at positions 4-8. NGG PAM requirement. Dual-AAV packaging required.

Parts

AncBE4max bGH pA U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

  1. Koblan et al. (2018). Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol.

Available Products

Additional Information

Applications: In vivo therapeutic applications where editor stability at body temperature is critical. Long-duration editing experiments requiring sustained editor activity. Systemic delivery applications with extended circulation time. Tissues with elevated temperature or oxidative stress. When protein stability is limiting factor for editing efficiency.