U6-(gRNA)-(pro)-BE3-bGHpA

Express gRNA under U6 and BE3 under selected promoter for foundational cytosine base editing with broad validation

Characteristics

Original cytosine base editor with APOBEC1 deaminase fused to SpCas9 D10A nickase. Generates C-to-T conversions with editing window at protospacer positions 4-8. Extensively validated across cell types and organisms. NGG PAM requirement. Requires dual-AAV packaging due to SpCas9 size. Moderate indel frequency compared to newer variants.

Parts

BE3 bGH pA U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

  1. Komor et al. (2016). Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature.

Available Products

Additional Information

Applications: Well-characterized targets with established protocols and published guides. Research applications where extensive literature precedent is valuable. Gene knockout via introduction of premature stop codons. Splice site disruption for exon skipping. Use when validated BE3 data exists for your target locus.