Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Komor et al. (2016). Nature | DOI: 10.1038/nature17946 | Citations: 6436

Key Finding

First cytosine base editor (BE3) fusing rat APOBEC1 to Cas9 nickase with UGI. Achieved >30% C-to-T editing efficiency with only 1.1% average indel frequency across multiple human cell line targets.

Parts Used

BE3