dCas9 Catalytically Dead Nuclease

Length: 4100 bp(1366 aa)

PAM: NGG (binds but does not cut)

Origin: Streptococcus pyogenes (engineered)

Characteristics

Catalytically inactive Cas9 with D10A and H840A mutations abolishing nuclease activity. Retains DNA binding capability. Foundation for fusion proteins in epigenome editing, base editing, and transcriptional regulation. No DNA cleavage activity.

Literature References

  1. Mali et al. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol - Mali 2013 Cas9 Nickase

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