Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

Hilton et al. (2015). Nat Biotechnol DOI: 10.1038/nbt.3199 Citations: 2397

Key findings

Developed dCas9-p300 system for targeted histone acetylation. Created active chromatin marks (H3K27ac) at target enhancers and promoters. More durable gene activation than transcriptional activators alone.

Parts used

dCas9-p300 Histone Acetylation Nuclease dCas9 Catalytically Dead Nuclease p300 Core Domain