dCas9-p300 Histone Acetylation Nuclease

Architecture

Catalytically dead Cas9 fused to catalytic core of human p300 histone acetyltransferase. Deposits acetyl groups on histone H3 lysine 27 (H3K27ac) at target enhancers and promoters.

Characteristics

Targeted histone acetylation for gene activation through chromatin remodeling. Creates active chromatin marks at target loci. More durable activation than VP64-based systems. Particularly effective for activating developmental genes and endogenous enhancers.

Literature References

1. Hilton et al. (2015). Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol - Hilton 2015 p300
2. Mali et al. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol - Mali 2013 Cas9 Nickase