CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

Mali et al. (2013). Nat Biotechnol DOI: 10.1038/nbt.2675 Citations: 1800

Parts used

dCas9 Catalytically Dead Nuclease

Key findings

  • Demonstrated Cas9 D10A nickase for enhanced specificity via paired nicking. Showed dCas9 fusions for transcriptional regulation. Foundation for base editors and epigenome editors.