Efficient targeted DNA methylation with chimeric dCas9-Dnmt3a-Dnmt3L methyltransferase

Stepper et al. (2017). Nucleic Acids Res DOI: 10.1093/nar/gkw1112 Citations: 379

Key findings

Developed dCas9-DNMT3A system for targeted DNA methylation. Achieved stable CpG methylation at target loci with heritable silencing. Established targeted epigenetic editing through DNA modification.

Parts used

dCas9-DNMT3A DNA Methylation Nuclease dCas9 Catalytically Dead Nuclease DNMT3A Domain