Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions

Morita et al. (2016). Nat Biotechnol DOI: 10.1038/nbt.3658 Citations: 572

Key findings

Developed dCas9-TET1 system for targeted DNA demethylation. Removed methylation at target CpG islands to reactivate silenced genes. Demonstrated epigenetic reactivation through targeted demethylation.

Parts used

dCas9-TET1 DNA Demethylation NucleasedCas9 Catalytically Dead NucleaseTET1 Domain