TET1 Domain

Length: 1 bp

Class: DNA Demethylase

Origin: Human ten-eleven translocation 1 (catalytic domain)

Characteristics

Targeted epigenetic activation through DNA demethylation. Removes stable methylation marks at target loci. Effective for reactivating epigenetically silenced genes. Larger domain (~500 amino acids for catalytic region). Slower-acting than transcriptional activators but creates lasting changes.

Mechanism: Catalyzes oxidation of 5-methylcytosine to 5-hydroxymethylcytosine, initiating active demethylation pathway. Removes repressive methylation marks to restore gene expression. Works through base excision repair pathway.

References

  1. Morita et al. (2016). Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Nat Biotechnol - Morita 2016 TET1

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