dCas9-TET1 DNA Demethylation Nuclease

Length: 1 bp(0 aa)

Function: DNA Demethylation

Base protein: dCas9 Catalytically Dead Nuclease

Architecture

Catalytically dead Cas9 fused to catalytic domain of ten-eleven translocation 1 (TET1) enzyme. Converts 5-methylcytosine to 5-hydroxymethylcytosine, initiating demethylation pathway.

Characteristics

Targeted DNA demethylation system for epigenetic activation. Removes methylation marks at target loci to reactivate silenced genes. Effective at reversing promoter methylation. Can restore expression of epigenetically silenced tumor suppressors or developmental genes.

Literature References

  1. Hilton et al. (2015). Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol - Hilton 2015 p300
  2. Morita et al. (2016). Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Nat Biotechnol - Morita 2016 TET1

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