Highly efficient Cas9-mediated transcriptional programming

Chavez et al. (2015). Nat Methods DOI: 10.1038/nmeth.3312 Citations: 1940

Key findings

Developed VPR tripartite activator combining VP64, p65, and Rta for enhanced CRISPRa. Achieved 10-1000× stronger activation than VP64 alone. Established VPR as gold standard for maximum gene activation.

Parts used

VPR Composite DomainVP64 Domainp65 DomainRta Domain