p65 Domain

Length: 1 bp

Class: Transcriptional Activator

Origin: Human NF-κB p65 subunit (activation domain)

Mechanism

Transcriptional activation domain from NF-κB p65. Recruits transcriptional coactivators and chromatin remodeling complexes. Synergizes with other activation domains in multi-domain fusions.

Characteristics

Strong transcriptional activator used in combination systems (e.g., VPR). More potent than VP64 alone but larger. Commonly combined with VP64 and Rta for enhanced activation. Particularly effective for difficult-to-activate genes.

Literature References

  1. Chavez et al. (2015). Highly efficient Cas9-mediated transcriptional programming. Nat Methods - Chavez 2015 VPR

Related collections

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