VP64 Domain

Length: 1 bp

Class: Transcriptional Activator

Origin: Herpes simplex virus VP16 (tetrameric repeat)

Mechanism

Tetrameric fusion of minimal VP16 activation domain. Recruits RNA polymerase II and transcriptional machinery to target promoters. Activates transcription through direct interaction with transcriptional coactivators.

Characteristics

Standard transcriptional activation domain for CRISPRa. Moderate activation strength (2-100× upregulation typical). Compact size (~50 amino acids). Well-characterized with minimal toxicity. Works across diverse cell types and promoter contexts.

Literature References

  1. Mali et al. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol - Mali 2013 Cas9 Nickase

Related collections

1 4