A protein-tagging system for signal amplification in gene expression and fluorescence imaging

Tanenbaum et al. (2014). Cell DOI: 10.1016/j.cell.2014.09.039 Citations: 1922

Key findings

Developed SunTag system using repeating GCN4 epitopes to recruit multiple scFv-effector fusions. Achieved 10-25× signal amplification. Applied to CRISPRa for ultra-strong gene activation (100-10,000× upregulation).

Parts used

SunTag-dCas9 Amplified Activation Nuclease dCas9 Catalytically Dead Nuclease GCN4 Domain