Custom Adenine Base Editor (Dual AAV)
Custom Adenine Base Editor (Dual AAV)
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Precision A-to-G base editing via dual-AAV split-intein delivery for in vivo applications. SpCas9-based ABE7.10 system reconstitutes in target cells from co-transduced N-terminal and C-terminal AAV vectors. Ideal for liver, muscle, and CNS applications requiring adenine editing with established SpCas9 PAM compatibility (NGG) and broad targeting scope. Alternative to single-AAV compact editors when NGG PAM access is essential.
- ABE N-term: N-terminal fragment of ABE7.10 split via Npu intein at E573. Requires C-terminal counterpart for reconstitution.
- ABE C-term: C-terminal fragment of ABE7.10 split via Npu intein at C574. Pairs with N-terminal fragment for editor reconstitution.
- EFS Promoter: Compact promoter derived from human elongation factor 1-alpha. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
Deliverables
- Titer: 1e13 VG/mL or higher
- Full Capsid Ratio: 0.85 or higher
- Delivery Timeline: 18–21 days
Specifications
Single stranded AAV produced in 293T cells and purified through two (2) rounds of CsCl ultracentrifugation and stored in 0.001% F-68 in DPBS with additional 150 mM NaCl. Ships on dry ice overnight.
This construct can be packaged in hundreds of AAV serotypes to target your specific tissue. We offer all standard serotypes (AAV1-9) plus specialized variants for muscle (MyoAAV-2A, MyoAAV-1A, AAVMyo), brain (PHP.eB, AAV9P31, AAV2-retro), and clinical applications (AAV2, Spark100). Other popular options include AAV-rh.74, AAV6, AAV6.2FF, and AAV3. Browse our complete serotype library or contact us for recommendations.
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