Adenine Base Editor N-terminal Fragment

N-terminal fragment of ABE7.10 split via Npu intein at E573. Requires C-terminal counterpart for reconstitution.

Length: 1000 bp (333 aa)

Type: Adenine Base Editor

Conversion: A-to-G

Origin: TadA-TadA* dimer fused to SpCas9 D10A nickase (amino acids 1-573)

Characteristics

Contains evolved TadA-TadA* adenine deaminase domain and N-terminal portion of SpCas9 D10A (residues 1-573). Split site at E573 positions Npu intein for efficient protein trans-splicing. Expresses from Cbh promoter in reference AAV construct. No sgRNA cassette in N-terminal fragment.

Applications

Dual-delivery base editing systems requiring split architecture. Validated for AAV delivery to brain, liver, retina, heart, and skeletal muscle. Compatible with alternative delivery methods including lentivirus or electroporation when paired with C-terminal fragment.

Limitations

Non-functional without C-terminal pair. Reconstitution efficiency depends on expression levels, intein splicing kinetics, and delivery method. Must be co-delivered with split-abe-ct for A-to-G editing activity.

Literature References

  1. Gaudelli NM, Komor AC, Rees HA, et al. (2017). Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464-471 - Gaudelli 2017 Adenine Base Editor
  2. Levy et al. (2020). Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng - Levy 2020 BE3.9max