U6-(gRNA)-(pro)-ABE-NT-bGHpA + EFS-ABE-CT-bGHpA

Two-vector system delivering ABE7.10 N-terminal and C-terminal split-intein fragments for reconstituted adenine base editing

Characteristics

First AAV delivers ABE N-terminal (TadA-TadA* + SpCas9 aa1-573) under EFS promoter. Second AAV delivers ABE C-terminal (SpCas9 aa574-1368) under selected promoter with U6-gRNA and BsmBI cloning site. Split at E573/C574 using DnaE intein. Reconstitutes full ABE7.10 activity in target cells. NGG PAM, editing window positions 4-8.

Parts

ABE N-term ABE C-term EFS Promoter bGH PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

  1. Gaudelli NM, Komor AC, Rees HA, et al. (2017). Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464-471- Gaudelli 2017 Adenine Base Editor

Available Products

No products currently available with this design. Contact us for custom orders.

Additional Information

Applications: In vivo adenine base editing when dual-AAV co-transduction is feasible. Liver, muscle, CNS applications with established dual-AAV efficiency. When SpCas9 PAM compatibility and editing window are required for therapeutic target. Alternative to single-AAV compact editors when NGG PAM access is critical.

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