Custom C-to-G Base Editor (Plasmid)
Custom C-to-G Base Editor (Plasmid)
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Direct C-to-G transversion editing in plasmid format for applications inaccessible to standard base editors. Enables unique C-to-G and G-to-C conversions that cannot be achieved through C-to-T or A-to-G pathways. Ideal for correcting transversion mutations, restoring specific codons, engineering gain-of-function variants, or exploring novel editing outcomes. Flexible plasmid delivery for screening and optimization.
Payload
- CGBE Base Editor: C-to-G transversion base editor expanding beyond transition mutations. Enables targeted C-to-G conversion addressing 11-40% of disease SNPs.
- bGH PolyA Signal: Compact (225 bp) polyadenylation signal from bovine growth hormone with 3-fold higher expression than SV40 early polyA in key mammalian cell types. Size advantage over SV40 polyA makes it the preferred terminator for AAV vectors with limited cargo space. Standard terminator in commercial gene therapy and lentiviral vector platforms with decades of in vivo validation.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
Specifications
High-copy plasmid DNA produced in E. coli with pMB1 origin of replication and ampicillin resistance marker. Purified by endotoxin-reduced anion-exchange chromatography (PureLink Maxi) and resuspended in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). Purity confirmed by A260/A280 spectrophotometry (≥1.8). Identity confirmed by Sanger or long-read sequencing. Yield ≥500 µg. Store at −20°C. Avoid repeated freeze-thaw cycles.
Deliverables
- Plasmid DNA at ≥500 µg, endotoxin-reduced maxiprep
- Payload sequence file
- QC report (A260/A280 ratio, concentration, restriction digest or sequencing confirmation)
- Ships on dry ice
References
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