Custom C-to-G Base Editor (Plasmid)
Custom C-to-G Base Editor (Plasmid)
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Direct C-to-G transversion editing in plasmid format for applications inaccessible to standard base editors. Enables unique C-to-G and G-to-C conversions that cannot be achieved through C-to-T or A-to-G pathways. Ideal for correcting transversion mutations, restoring specific codons, engineering gain-of-function variants, or exploring novel editing outcomes. Flexible plasmid delivery for screening and optimization.
- CGBE: C-to-G transversion base editor expanding beyond transition mutations. Enables targeted C-to-G conversion addressing 11-40% of disease SNPs.
- bGH pA: Compact and highly efficient polyadenylation signal from bovine growth hormone gene, widely used in AAV and size-constrained vectors.
- U6 Promoter: RNA polymerase III promoter that drives constitutive expression of small RNAs. Provides strong, stable transcription with precise transcriptional start and termination sites.
Deliverables
- Titer: 1 µg/mL or higher
- Delivery Timeline: 12–14 days
Specifications
High copy plasmid DNA produced in E. coli using the proprietary Ambryon backbone with pMB1 origin of replication and ampicillin resistance. Bacteria are cultured in LB medium at 37°C and harvested at late exponential phase. Cells are lysed by alkaline lysis and purified by endotoxin-free anion-exchange chromatography. Final DNA is resuspended in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) and quality-verified by A260/A280 spectrophotometry (≥1.8) and sequence confirmation. Store at -20°C and avoid repeated freeze-thaw cycles. Ships at ambient temperature.
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