U6-(gRNA)-(pro)-CGBE-bGHpA

Express gRNA under U6 and CGBE under selected promoter for direct C-to-G transversion editing

Characteristics

Enables direct C-to-G base transversions inaccessible to standard cytosine or adenine base editors. Also generates G-to-C conversions on complementary strand. Unique editing capability for applications requiring guanine installation at cytosine positions. Single-AAV compatible architecture. Editing window and efficiency vary by target context.

Parts

CGBE Base Editor bGH PolyA Signal U6 Promoter

Custom Inserts

SpCas9 gRNA target (20-23 bp)

Literature Sources

    Available Products

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    Additional Information

    Applications: Correcting C-to-G or G-to-C transversion mutations that cannot be addressed by C-to-T or A-to-G editors. Restoring specific codons requiring guanine at positions currently occupied by cytosine. Engineering gain-of-function variants. Research applications exploring transversion editing outcomes. When standard base editors cannot achieve desired nucleotide change.

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