SpCas9-HF1 Nuclease

Length: 4100 bp(1366 aa)

PAM: NGG

Origin: Streptococcus pyogenes (engineered)

Characteristics

High-fidelity SpCas9 variant with reduced off-target activity. Four point mutations weaken non-specific DNA contacts. Maintains on-target efficiency while dramatically improving specificity. Requires dual-AAV for in vivo use.

Literature References

  1. Kleinstiver et al. (2016). High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature - Kleinstiver 2016 SpCas9-HF1

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